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. 2014 Jul 16;34(29):9607–9620. doi: 10.1523/JNEUROSCI.3788-13.2014

Figure 1.

Figure 1.

TFEB stimulates lysosome biogenesis and function. A, Representative confocal images demonstrating LysoTracker Red expression in Cerulean-tagged TFEB-transfected (Cer-TFEB) C17.2 cells versus empty vector transfected controls. Representative of n = 4 independent experiments. B, Representative confocal images demonstrating LAMP1 expression in C17.2 cells transfected with FLAG-tagged TFEB versus empty vector transfected controls. Representative of n = 4 experiments. C, Flow cytometric analysis of LysoTracker Red staining in cells transfected with FLAG-tagged TFEB or empty vector (V; as control), with quantification of mean fluorescence expressed as fold over control (inset). N = 3/group. D, E, Immunoblots (D) and quantification (E) of LAMP1 and Cathepsin B and D expression in cells transfected as in C. F, Cathepsin B and D activity in cells transfected as in C. N = 4/group; **p < 0.01.