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. 2014 Jul 16;34(29):9607–9620. doi: 10.1523/JNEUROSCI.3788-13.2014

Figure 4.

Figure 4.

AAV8-mediated gene transfer of GFAP-promoter driven TFEB targets expression specifically to astrocytes. A, Representative fluorescence images of GFP expression in the hippocampus of APP/PS1 mice injected with AAV8-GFAP-eGFP viral particles. Sequential images of brain sections confirm high transduction efficiency throughout the anterior hippocampus. B, Representative confocal images demonstrating expression of TFEB (green) with GFAP (red, top), Iba-1 (red, middle), and NeuN (red, bottom) in the hippocampus of APP/PS1 mice injected with AAV8-GFAP-FLAG-TFEB particles, demonstrating astrocyte-specific expression of TFEB. The boxed inserts (upper right corner) demonstrate magnified images of individual TFEB-labeled cells (arrows). DG, dentate gyrus. C, D, Immunoblot and quantitation of APP, α-CTF, and β-CTF in AAV8-GFAP-FLAG-TFEB and AAV8-GFAP-eGFP transduced hippocampi. N = 3/group. E, Neuronal counts in the CA1 and CA3 layers of the hippocampi from AAV8-GFAP-FLAG-TFEB and AAV8-GFAP-eGFP transduced APP/PS1 mice and uninjected age- and sex-matched APP/PS1 mice as controls. N = 4/group.