Figure 6.

Ibuprofen-CPP is independent of a leptin-glia crosstalk in NAc. A, Leptin (ob) levels in the NAc were not increased in sham or arthritic rats exposed to ibuprofen conditioning. B, Intra-NAc microinjection of LA (4 μg; once daily × 5 d) failed to reduce the CPP score in arthritic rats exposed to ibuprofen conditioning (CFA/Ibu/LA; n = 6). Intra-NAc LA alone did not change the baseline CPP score in sham rats exposed to ibuprofen conditioning (Ibu/LA; n = 6); *p < 0.05 compared with S/V. C–E, GFAP expression in the NAc (C) was downregulated, whereas Th (D), D1, and D2 (E) expression was upregulated, in CFA/Ibu; β-Actin: loading control; *p < 0.05, **p < 0.01, ***p < 0.001 compared with S/V. Data are shown as mean + SD. F, Location of microinjections into the NAc (AP: 1.7 mm from bregma; left: 2.2 mm; depth: 6.7 mm).