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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Am J Physiol. 1999 Jan;276(1 0 1):C102–C108. doi: 10.1152/ajpcell.1999.276.1.C102

Fig. 8.

Fig. 8

Effect of T3 on 5′-flanking region of the NHE3 gene. Two chloramphenicol acetyltransferase (CAT) reporter constructs containing either 2.2 or 0.82 kb of the 5′-flanking region of the rat NHE3 gene were transiently transfected into OK cells along with a β-galactosidase-expressing plasmid. OK cells were treated with 10−7 M T3 or vehicle for 4 h, and CAT activity was assayed in cell lysates with equivalent amounts of β-galactosidase activity. Four (for 0.82 kb) and 2 (for 2.2 kb) independent experiments showed similar results.