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. 2014 Jul 16;5:330. doi: 10.3389/fpls.2014.00330

Figure 2.

Figure 2

BAC2 overexpression and amino acid content of 8-day-old Arabidopsis seedlings. (A) PCR on genomic DNA of Arabidopsis Col-0, bac2-1, p35S:BAC2 in bac2-1 genetic background (named bac2-1, BAC2-OE) p35S:BAC2 in Col-0 (named BAC2-OE #1 and BAC2-OE #2). Arrows indicate BAC2 genomic DNA and cDNA sizes. ACT2 was used as a control of genomic DNA quality. (B) Semi-quantitative RT-PCR. BAC2 expression levels were detected in Arabidopsis Col-0, bac2-1, and p35S:BAC2 in bac2-1 (named bac2-1, BAC2-OE) seedlings non-treated (NT) or treated with 0.4 M mannitol (T). ACT2 gene expression level was used as a control. (C) Values in nanomoles of amino acid per gram of fresh weight of total plant tissue. Col-0 is the WT control, bac2-1 is the KO mutant, and BAC2-OE is an overexpressor line (BAC2-OE #2). Only amino acids which differ significantly between BAC2-OE and Col-0 are shown. Arg, arginine; Cit, citrulline; Orn, ornithine; Pro, proline; Ala, alanine. Letters refer to statistically different values significant at p < 0.05 (n = 3) in the Student t-test.