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. 2014 May 8;5(2):58–77. doi: 10.3390/jfb5020058

Figure 9.

Figure 9

Western blot analysis of the expression of (A) CAL and (B) MYH on different surfaces after seven, 14 and 21 days in culture. Experiments were replicated with western blots with at least three different cell populations (nmin = 9). Statistical significance was calculated using a one-way ANOVA with Tukey’s post hoc test. After seven days in culture, NW and colNW express significantly more amounts of CAL and MYH compared to the PCL and colPCL surfaces, while the NW surfaces express CAL and MYH significantly more than colNW. After 14 days in culture, CAL expression is significantly higher on the colNW surfaces compared to the PCL and NW surfaces. After 14 days in culture, both collagen immobilized surfaces (colPCL and colNW) express significantly more MYH than the PCL and NW surfaces, while the PCL surfaces express significantly more MYH than the NW surfaces. After 21 days in culture, CAL and MYH expression is elevated significantly on the colPCL and colNW surfaces compared to the PCL and NW surfaces.