Table 2. Validation of in vivo differential expression from transcriptional profile of Acidovorax avenae subsp. avenae strain RS-1 by the changes in pathogenicity of T6SS gene mutants constructed in this study.

		NGEVa of RNA-Seq		Validation by mutation
Locus Tag	T6SS components	In vitro	In vivo	Gene	Pathogenicity
Acav_1267	ClpB	5.9	2	ΔclpB	loss
Acav_1504	Hcp	123.6	24.85	Δhcp	loss
Acav_1905	VgrG-8	11.95	5	ΔvgrG	loss
Acav_1512	IcmF	6.4	1.8	ΔicmF	loss
Acav_1519	DUF879	5.85	2.6	ΔdotU	loss
Acav_1511	DotU/OmpA/MotB	11.05	4.4	ΔompA	loss
Acav_2399	VgrG-3	4.05	0	ΔimpA	loss
Acav_1516	Duf877/EvpB	68.35	22.55	ΔevpB	decrease
Acav_0662	VgrG-2	13.7	0	ΔvgrG	loss
Acav_0298	VgrG-1	1.65	3.7	ΔvgrG	loss
Acav_1513	ImpM	4.85	1.9	ΔimpM	loss
Acav_4620	PppA	8.95	6.1	ΔpppA	Not loss
Acav_1509	Lip	12.65	3.55	Δlip	Not loss

^aNGEV: Normalized gene expression value. The detailed knockout of these T6SS genes and their pathogenic function will be published in another paper.