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Two-dimensional gels from human plasma samples. (A) Native human plasma (total protein 50 µg, pH 4–7, 12.5% SDS-PAGE). (B) Human plasma filtered with 30-kDa cut-off filters (total protein 50 µg, pH 3–10, 15% SDS-PAGE). (C) Human plasma treated with 20% ACN, 25 mM NH4HCO3 and filtered with 30 kDa and 3 kDa cut-off filters (total protein 22 µg, pH 3–10, 15% SDS-PAGE).
