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. 2014 Jul 16;5:232. doi: 10.3389/fgene.2014.00232

FIGURE 4.

FIGURE 4

Modulation of BER through Mule-dependent regulation of Pol β protein levels, as revealed by the in-gel DNA repair comet assay. HeLa cells (A,C) or WI-38 cells (B,D) were treated with Lipofectamine transfection reagent (Life Technologies, Paisley, UK) in the absence (Mock siRNA) and presence of Mule siRNA (A,B) or ARF siRNA (C,D) for 72 h. Cells were analyzed using the in-gel DNA repair comet assay following treatment in suspension with 20 μM hydrogen peroxide for 5 min and allowing for DNA damage repair at 37∘C for up to 120 min. The mean % tail DNA values with SDs from at least three independent experiments were determined using the Komet 5.5 image analysis software (Andor Technology, Belfast, UK). Statistically significant results comparing Lipofectamine and siRNA-treated cells are represented by *p < 0.02, **p < 0.005, and ***p < 0.001, as analyzed by Student’s t-test. Data taken and modified from Parsons et al. (2009).