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. 2014 Jul 16;5:232. doi: 10.3389/fgene.2014.00232

FIGURE 6.

FIGURE 6

Modulation of BER through Cul4A–DDB1–STRAP- dependent regulation of PNKP protein levels, as revealed by the in-gel DNA repair comet assay. HCT116p53+/+ cells were treated in the absence or presence of ATM siRNA for 24 h. Cells were then further treated in the absence (Mock siRNA and ATM siRNA) and presence (ATM siRNA + PNKP) of a mammalian expression plasmid expressing Flag-tagged PNKP for a further 24 h. Cells were analyzed using the in-gel DNA repair comet assay following treatment in suspension with 35 μM hydrogen peroxide for 5 min, and allowing for DNA damage repair. Mean % tail DNA values with SDs were calculated. Further details are provided in Figure 4 legend. Statistically significant results comparing ATM siRNA versus ATM siRNA-treated cells transfected with a plasmid containing Flag-tagged PNKP are represented by *p < 0.0001, as analyzed by Student’s t-test. Data taken and modified from Parsons et al. (2012).