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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Cell Rep. 2014 Jun 5;7(6):1887–1899. doi: 10.1016/j.celrep.2014.05.019

Figure 5. Combined inhibition of DDX5 and BCL2 family proteins cooperate to induce apoptosis in THP1 AML cells.

Figure 5

(A) Immuno-blot analysis of BCL2 family proteins in WCEs from the indicated AML cell lines. Note WCEs prepared from 50,000 cells per cell line were loaded onto the immuno-blots and results for triplicate independently prepared WCEs from each cell line are presented. (B) Immuno-blot analysis of Caspase 3 activation for THP1 cells +/− DDX5 knockdown treated for 24hrs with increasing concentration of ABT-737. Concentrations of ABT-737 tested were 0µM, 0.1µM, 0.5µM, 1µM, 5µM, and 10µM. (C) Flow cytometry analysis of live GFP positive cells in THP1 cultures +/− DDX5 knockdown untreated or treated for 24hrs with either low dose (0.5µM) or high dose (5µM) ABT-737. Results from duplicate cultures per RNAi condition are presented. GFP positive cells were gated and the percent of GFP positive cells in each culture are shown.