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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Cell Rep. 2014 Jun 5;7(6):1887–1899. doi: 10.1016/j.celrep.2014.05.019

Figure 6. DDX5 knockdown in adult mice is well tolerated.

Figure 6

(A) Immuno-blot analysis of the indicated proteins in WCEs prepared from the noted organs of duplicate mice either treated (ON-DOX-lanes 1 and 2) or untreated (OFFDOX-lanes 3 and 4) with doxycycline to induce shRNA expression. WCEs prepared from the organs of the fourth OFF-DOX mouse is loaded on each blot either with equal total protein loading as the WCEs from the other mice (lane 4) or they were diluted either 1-to-2 (lane 5), 1-to-4 (lane 6) or 1-to-10 (lane 7). (B) Representative tissue sections obtained from mice +/− doxycycline stained to detect DDX5 protein. The brown staining marks DDX5 protein in the tissues. For the ON-DOX mice presented in both (A) and (B) the organs were dissected after 2 weeks of continuous doxycycline to induce shRNA expression. (C) Weight measurements of mice either given or not given doxycycline that were measured over 2 months. Data is presented as the mean from multiple mice (n=6 for each genotype/doxycycline group) with standard deviations shown. (D) Flow cytometry analysis of GFP positive DDX5 shRNA expressing peripheral blood leukocytes at either 1 week or 5 weeks of continuous doxycycline administration. Data is presented as the mean from duplicate mice with standard deviations shown.