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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Cell Rep. 2014 Jun 5;7(6):1887–1899. doi: 10.1016/j.celrep.2014.05.019

Figure 7. DDX5 depletion does not impair mouse recovery from stress caused by high dose irradiation.

Figure 7

(A) Representative tissue sections obtained from mice +/− doxycycline +/− irradiation with 550Rad IR. Tissue samples were collected 4hrs after irradiation and stained for the indicated proteins. (B) Immuno-blot analysis of WCEs prepared from bone marrows of mice +/− doxycycline +/− irradiation (4hrs post-IR). (C) Weight measurements of mice with the indicated genotypes +/− doxycycline +/− irradiation every other day for 1 month following IR. Results for irradiated mice are indicated by “X” labels and results for non-irradiated mice are indicated with closed squares. Results for shDDX5.2086 mice ondoxycycline are color coded red, results for shDDX5.2086 mice off-doxycycline are color coded blue, and results for No shRNA mice are color coded green. Data is presented as the mean from multiple mice (n=4 for each genotype +/− doxycycline +/− irradiation) with standard deviations shown. (D) Quantitative immuno-blot analysis of DDX5 in WCEs prepared from bone marrow collected from duplicate mice +/− doxycycline +/− irradiation at either 4hrs after IR (upper blot) or 28 days after IR (lower blot). In both blots, bone marrow WCE from the second irradiated mouse that was not given doxycycline was loaded at either equivalent total protein as the other bone marrow WCEs (lane 8) or was diluted either 1-to-2 (lane 9), 1-to-4 (lane 10) or 1-to-10 (lane 11).