Table 1.
Inhibitory effects of compounds 1–17 from the fruits of C. monnieri on superoxide radical anion generation and elastase release by human neutrophils in response to fMet-Leu-Phe/cytochalasin B a.
| Compounds | Superoxide Anion | Elastase |
|---|---|---|
| IC50 [µg/mL] b or (Inh %) c | ||
| 3'-O-Methylmurraol (1) | 6.24 ± 0.50 g | (35.10 ± 5.71) f |
| 1'-O-Methylphlojodicarpin (2) | (15.04 ± 1.26) g | (6.02 ± 1.56) e |
| 1'-O-Methylvaginol (3) | (11.99 ± 2.14) f | (6.42 ± 2.07) e |
| Murraol (4) | 2.83 ± 0.46 f | (18.16 ± 5.62) e |
| Peroxymurraol (5) | 2.87 ± 0.11 g | (5.78 ± 2.98) |
| Osthol (6) | 0.005 ± 0.0002 f | (31.96 ± 5.72) f |
| Osthenol (7) | 0.09 ± 0.01 g | 3.28 ± 0.90 g |
| Auraptenol (8) | 0.77 ± 0.11 e | (22.46 ± 2.65) f |
| Peroxyauraptenol (9) | 0.41 ± 0.06 e | 7.83 ± 1.17 e |
| Meranzin hydrate (10) | 7.31 ±1.62 e | 4.21 ± 1.41 e |
| Demethylauraptenol (11) | 0.54 ± 0.05 g | 4.36 ± 1.67 f |
| Xanthotoxin (12) | 0.32 ± 0.13 e | (14.81 ± 6.03) |
| Xanthotoxol (13) | (24.54 ± 2.47) g | (42.94 ± 4.29) g |
| Imperatorin (14) | 0.07 ± 0.02 g | (22.08 ± 2.93) f |
| Bergapten (15) | 0.36 ± 0.09 e | 4.62 ± 1.36 e |
| Isopimpinellin (16) | 2.75 ± 0.26 | (8.97 ± 2.28) e |
| Cnidimol A (17) | 3.65 ± 0.41 g | 3.20 ± 0.16 g |
| Diphenyleneiodonium d | 0.55 ± 0.20 g | - |
| Phenylmethylsulfonyl fluoride d | - | 34.4 ± 5.5 g |
a Results are presented as averages ± SEM (n = 4); b Concentration necessary for 50% inhibition (IC50). If IC50 value of compound was <10 µg/mL, it was displayed as IC50 [µg/mL]; c Percentage of inhibition (Inh %) at 10 µg/mL. If IC50 value of compound was ≥10 µg/mL, it was shown as (Inh %) at 10 µg/mL; d Diphenyleneiodonium and phenylmethylsulfonyl were used as positive controls for superoxide anion generation and elastase release, respectively; e p < 0.05 compared with the control; f p < 0.01 compared with the control; g p < 0.001 compared with the control.