Table 2.
Identified proteins differentially expressed during flowering.
| Spot a | Ratio to C1F b | Matched Protein c | Accession Number d | Cov. % e | MW f | Functional Category | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C2F | DsF | NpF | |||||||||||||||
| 3015 | 4.56 | 4.69 | 2.95 | thioredoxin H-type isoform 1 (Vitis vinifera) | gi|225458147 | 62 | 12.80 | Protein folding | |||||||||
| 3014 | 2.80 | 26.5 | 1.78 | 18.2 kDa class I heat shock protein isoform 1 (Vitis vinifera) | gi|225449302 | 46 | 17.02 | Protein destination | |||||||||
| 2123 | 0.83 | 3.52 | 1.25 | 23.6 kDa heat shock protein, mitochondrial isoform 1(Vitis vinifera) | gi|225466111 | 42 | 23.74 | Protein destination | |||||||||
| 1009 | 0.58 | 0.96 | 3.47 | 18.2 kDa class I heat shock protein isoform 1 (Vitis vinifera) | gi|225449302 | 13 | 17.02 | Protein destination | |||||||||
| 6222 | 1.04 | 0.61 | 3.05 | l-ascorbate peroxidase 2, cytosolic (Vitis vinifera) | gi|225435177 | 65 | 27.56 | Carbohydrate metabolism | |||||||||
| 6222 | 1.04 | 0.61 | 3.05 | triosephosphate isomerase, cytosolic (Vitis vinifera) | gi|225449541 | 53 | 21.13 | Glycolysis/Gluconeogenesis | |||||||||
| 2409 | 0.70 | 1.37 | 2.10 | l-galactose dehydrogenase (Vitis vinifera) | gi|146432259 | 47 | 34.64 | Carbohydrate metabolism | |||||||||
| 2409 | 0.70 | 1.37 | 2.10 | pyruvate dehydrogenase E1 component subunit beta, mitochondrial-like isoform 1 (Vitis vinifera) | gi|225425166 | 42 | 39.49 | Glycolysis/Gluconeogenesis | |||||||||
| 2409 | 0.70 | 1.37 | 2.10 | PREDICTED: fructokinase-2 (Vitis vinifera) | gi|225433918 | 53 | 35.20 | Carbohydrate metabolism | |||||||||
| 2409 | 0.70 | 1.37 | 2.10 | isoflavone reductase homolog P3 (Vitis vinifera) | gi|225458243 | 36 | 33.81 | Secondary metabolism | |||||||||
| 5016 | 1.08 | 3.31 | 0.46 | 18.2 kDa class I heat shock protein (Vitis vinifera) | gi|225449250 | 49 | 18.15 | Protein destination | |||||||||
| 5016 | 1.08 | 3.31 | 0.46 | pathogenesis-related protein 10 (Vitis hybrid cultivar) | gi|163914213 | 14 | 17.11 | Defense and cell rescue | |||||||||
| 4627 | 0.43 | 1.08 | 1.38 | MLP-like protein 34 (Vitis vinifera) | gi|225424277 | 47 | 17.08 | Defense and cell rescue | |||||||||
| 4627 | 0.43 | 1.08 | 1.38 | chaperonin CPN60-2, mitochondrial isoform 1 (Vitis vinifera) | gi|225433375 | 45 | 61.37 | Protein destination | |||||||||
| 4627 | 0.43 | 1.08 | 1.38 | ruBisCO large subunit-binding protein subunit beta, chloroplastic (Vitis vinifera) | gi|225435794 | 42 | 64.61 | Photosynthesis | |||||||||
| 6018 | 1.90 | 0.24 | 1.12 | ubiquitin-conjugating enzyme E2 35 isoform 1 (Vitis vinifera) | gi|225461646 | 51 | 17.22 | Protein degradation | |||||||||
| 6018 | 1.90 | 0.24 | 1.12 | MLP-like protein 34 (Vitis vinifera) | gi|225424277 | 72 | 17.08 | Defense and cell rescue | |||||||||
| 6018 | 1.90 | 0.24 | 1.12 | chaperonin CPN60-2, mitochondrial isoform 1 (Vitis vinifera) | gi|225433375 | 27 | 61.37 | Protein destination | |||||||||
| 6018 | 1.90 | 0.24 | 1.12 | ubiquitin-conjugating enzyme E2 36 isoform 1 (Vitis vinifera) | gi|225446595 | 38 | 17.22 | Protein degradation | |||||||||
| 5018 | 1.65 | 0.17 | 0.65 | MLP-like protein 34 (Vitis vinifera) | gi|225424277 | 76 | 17.08 | Defense and cell rescue | |||||||||
| 5018 | 1.65 | 0.17 | 0.65 | ubiquitin-conjugating enzyme E2 36 isoform 1 (Vitis vinifera) | gi|225446595 | 38 | 17.22 | Protein degradation | |||||||||
| 5018 | 1.65 | 0.17 | 0.65 | glycine-rich RNA-binding protein GRP1A-like (Vitis vinifera) | gi|359475330 | 17 | 16.33 | Cell growth and death | |||||||||
| 5018 | 1.65 | 0.17 | 0.65 | superoxide dismutase (Cu-Zn) isoform 2 (Vitis vinifera) | gi|225451120 | 29 | 15.28 | Defense and cell rescue | |||||||||
| 5216 | 0.73 | 0.50 | 0.42 | putative transcription factor (Vitis vinifera) | gi|14582465 | 31 | 16.70 | Protein synthesis | |||||||||
| 5216 | 0.73 | 0.50 | 0.42 | ferritin-3, chloroplastic (Vitis vinifera) | gi|147784301 | 18 | 25.37 | Metabolism of cofactors and vitamins | |||||||||
| 5216 | 0.73 | 0.50 | 0.42 | triosephosphate isomerase, chloroplastic-like isoform 1 (Vitis vinifera) | gi|225427917 | 59 | 34.67 | Glycolysis/Gluconeogenesis | |||||||||
| 4421 | 0.52 | 0.47 | 0.33 | glutelin type-A 1 (Vitis vinifera) | gi|225435090 | 44 | 38.33 | - | |||||||||
| 4421 | 0.52 | 0.47 | 0.33 | glutamine synthetase nodule isozyme isoform 1 (Vitis vinifera) | gi|225451235 | 19 | 34.37 | Nitrogen metabolism | |||||||||
| 5217 | 0.61 | 0.32 | 0.19 | stem-specific protein TSJT1 (Vitis vinifera) | gi|225432548 | 46 | 25.25 | Defense and cell rescue | |||||||||
| 0207 | 0.20 | 0.10 | 0.24 | putative ripening-related protein (Vitis vinifera) | gi|7406667 | 23 | 15.39 | - | |||||||||
| 0309 | 0.35 | 0.24 | 0.16 | uncharacterized protein LOC100232885 (Vitis vinifera) | gi|225447003 | 78 | 18.41 | - | |||||||||
a spot code as reported in Figure 3; b ratio of spot expression values (relative OD × area%) in C2, Ds and Np stems to the related control (C1G, C1F or C1V). Values indicating over or down expression (ratio ≥ |2|) are highlighted in yellow or grey, respectively. Values were replaced by an asterisk when the spot was not detected in the control; c protein identified via the MASCOT and OMSSA search engines against in house made database from NCBInr database; d accession No. of the matched protein as reported in the NCBI database; e percentage of the protein sequence covered by the matching peptides; f molecular mass (kDa).