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FRET efficiency and distance of the ROI from the cells co-transfected with pECFP-C1-SelP' and pEYFP-C1-Tub determined by receptor photobleaching method.
| Cell Number | FRET Efficiency E (%) | Distance between Donor and Acceptor r (nm) |
|---|---|---|
| 1 | 17.6 | 7.794 |
| 2 | 14.1 | 8.239 |
| 3 | 12.2 | 8.816 |
| 4 | 31.5 | 6.634 |
| 5 | 20.0 | 7.595 |
| 6 | 26.7 | 7.293 |
| 7 | 19.2 | 7.845 |
| 8 | 17.9 | 7.744 |
| 9 | 35.6 | 6.341 |
| 10 | 20.2 | 7.551 |
| 11 | 25.0 | 6.856 |
| Average | 21.81 | 7.519 |
