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. 2014 Jun 10;15(6):10215–10232. doi: 10.3390/ijms150610215

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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SDS-PAGE of the expressed product for pET 28a (+)-Bx-Prx in E. coli BL21(DE3). Lane 1: molecular weight markers (40, 25, 15, 10 kDa); Lane 2: soluble fraction of induced cells transformed by pET-28a; Lane 3: soluble fraction of induced cells transformed by pET 28a (+); Lane 4: inclusion body fraction of induced cells transformed by pET-28a and Lane 5: inclusion bodies fraction of induced cells transformed by pET-28a (+)-Bx-Prx; Lane 6: the purified pET 28a (+)-Bx-Prx recombinant protein.