Figure 3.

A-769662-induced pro-survival effect against H₂O₂ requires AMP-activated protein kinase (AMPK) activation. MG-63 and MC3T3-E1 cells were pre-treated with A-769662 (10 μM) for 1 h, followed by hydrogen peroxide (H₂O₂, 250 μM) stimulation, cells were further cultured for indicated time points, and western blots were performed to test phospho(p)- and total (t)-AMPKα, p- and t- ACC (A) and (B); MG-63 and MC3T3-E1 cells were pre-treated with AMPK inhibitor compound C (Cpd C, 10 μM) for 2 h, followed by hydrogen peroxide (H₂O₂, 250 μM) or H₂O₂+ A-769662 (10 μM) stimulation, cells were further cultured, cell viability (C) and Annexin V percentage (D) were tested as described; MG-63 and MC3T3-E1 cells were pre-treated with compound C (Cpd C, 10 μM) for 2 h, followed by AICAR (0.5 and 1 mM) stimulation, cells were further cultured for 72 h when cell viability was examined (E) and (F). # p < 0.05.