Fig. 2.
Distributions of the differences between CD4+ (A) and CD8= (B) T-cell percentages obtained by site 4 and the IQA medians on individual specimens, using the 2-color lyse-wash method (left) and the 3-color lyse no-wash method (right). Within each plot, differences for individual samples are shown on the left, and box plots of the differences are shown on the right (box plots defined as per legend to Fig. 1). To equalize sample size across methods, only the last eighteen 2-color samples were included for comparison with the 3-color results. Plots show improved IQA performance for the 3-color lyse no-wash method.