Table 2.
Differentially Methylated CpG Loci in Clinically defined Low and High-risk anal cancers
| Symbol | Product | Association with Disease | CpG Numbera | Median Beta value | p-valuec | |||
|---|---|---|---|---|---|---|---|---|
| Low Risk | High Risk | Difb | MW | T-Test | ||||
| ADAT3d | Adenosine deaminase, tRNA-specific 3 | Not reported as target of methylation or involved in cancer | cg20243900 | 0.64 | 0.75 | 0.11 | 5.48E-04 | 2.01E-04 |
| GSG1L | Germ Cell Associated 1 (GSG1)-Like | Possible susceptibility locus for colon cancer in AOM mouse models; Not reported as target of methylation. | cg09528825 | 0.07 | 0.18 | 0.11 | 3.12E-02 | 8.15E-04 |
| KIAA0319 | KIAA0319 | Dyslexia-associated gene, Not reported as target of methylation or involved in cancer | cg18428180 | 0.24 | 0.09 | −0.15 | 1.29E-03 | 4.87E-04 |
| LOC-728392 | LOC728392 | Not reported as target of methylation or involved in cancer | cg18671949 | 0.11 | 0.40 | 0.29 | 4.96E-05 | 9.84E-06 |
| cg06462347 | 0.24 | 0.53 | 0.28 | 1.43E-04 | 5.17E-05 | |||
| cg27230784 | 0.14 | 0.51 | 0.37 | 4.44E-05 | 4.58E-05 | |||
| cg22298430 | 0.15 | 0.47 | 0.32 | 1.49E-04 | 7.13E-05 | |||
| cg01433610 | 0.38 | 0.52 | 0.15 | 1.66E-03 | 8.04E-04 | |||
| PARD3 | Partitioning defective 3 homolog | Lost in prostate cancer23, involved in cell motility; Associated with WNT/β-catenin pathway24; Not reported as a target of methylation. | cg26795540 | 0.48 | 0.58 | 0.10 | 7.57E-04 | 1.60E-03 |
| SALL3 | SAL-Like 3 | Methylated in HCC25; interacts with DNMT3A to reduce methylation | cg13794993 | 0.22 | 0.38 | 0.17 | 6.89E-04 | 4.15E-04 |
| cg14007067 | 0.14 | 0.31 | 0.17 | 1.27E-03 | 8.36E-04 | |||
| cg06954658 | 0.16 | 0.27 | 0.11 | 1.47E-03 | 4.78E-04 | |||
| cg16433156 | 0.14 | 0.25 | 0.11 | 1.34E-03 | 5.46E-04 | |||
| cg05080154 | 0.11 | 0.29 | 0.18 | 2.36E-03 | 9.22E-04 | |||
| SFRP2 | secreted frizzled-related protein 2 | Methylated in several cancers, including cervical26; head and neck27, and prostate28 | cg22178613 | 0.16 | 0.38 | 0.22 | 1.10E-03 | 8.78E-04 |
| ST6GAL2 | ST6 beta-galactosamide alpha-2,6-sialyltranferase 2 | Upregulated in breast cancer29. Not reported as target of methylation. | cg13168617 | 0.72 | 0.57 | −0.15 | 1.75E-03 | 8.25E-04 |
| SCAMP4d | Secretory Carrier Membrane Protein 4 | Not reported as target of methylation or involved in cancer | cg20243900 | 0.64 | 0.75 | 0.11 | 5.48E-04 | 2.01E-04 |
CpG number is the unique identifier for the loci provided by Illumina; multiple loci within a differentially methylated region listed in descending order according to genomic position.
Difference in median β-value between high and low risk tumors
Statistical differences in β-values between the two groups were determined by Mann-Whitney or student's t-test
CpG loci located in a genomic sequence that overlaps for ADAT3 and SCAMP4 genes.