Table 2.
Amino acid composition and molecular weight of proteases across different families.
| Amino | CAQ76821 | CAG25549 | EDR11679 | CAG38357 | CAD11898 | CAL25578 | EDU50747 | AAW40780 | ACB30121 | ACB30119 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| acid | Qty | % | Qty | % | Qty | % | Qty | % | Qty | % | Qty | % | Qty | % | Qty | % | Qty | % | Qty | % | |
| Ala | (A) | 97 | 10.1 | 81 | 9.1 | 78 | 8.7 | 75 | 9.0 | 82 | 9.9 | 101 | 11.5 | 81 | 8.9 | 91 | 9.6 | 80 | 9.2 | 105 | 11.6 |
| Arg | (R) | 40 | 4.2 | 27 | 3.0 | 22 | 2.4 | 16 | 1.9 | 17 | 2.1 | 18 | 2.1 | 29 | 3.2 | 22 | 2.3 | 21 | 2.4 | 31 | 3.4 |
| Asn | (N) | 44 | 4.6 | 38 | 4.3 | 43 | 4.8 | 41 | 4.9 | 42 | 5.1 | 40 | 4.6 | 52 | 5.7 | 51 | 5.4 | 45 | 5.2 | 43 | 4.7 |
| Asp | (D) | 49 | 5.1 | 62 | 6.9 | 54 | 6.0 | 65 | 7.8 | 55 | 6.7 | 51 | 5.8 | 53 | 5.8 | 66 | 6.9 | 52 | 6.0 | 63 | 7.0 |
| Cys | (C) | 8 | 0.8 | 5 | 0.6 | 4 | 0.4 | 4 | 0.5 | 9 | 1.1 | 8 | 0.9 | 9 | 1.0 | 9 | 0.9 | 7 | 0.8 | 10 | 1.1 |
| Gln | (Q) | 23 | 2.4 | 34 | 3.8 | 23 | 2.6 | 28 | 3.4 | 30 | 3.6 | 37 | 4.2 | 35 | 3.9 | 29 | 3.1 | 19 | 2.2 | 26 | 2.9 |
| Glu | (E) | 21 | 2.2 | 16 | 1.8 | 19 | 2.1 | 20 | 2.4 | 15 | 1.8 | 20 | 2.3 | 25 | 2.8 | 26 | 2.7 | 28 | 3.2 | 24 | 2.6 |
| Gly | (G) | 100 | 10.4 | 89 | 10.0 | 84 | 9.3 | 85 | 10.2 | 85 | 10.3 | 77 | 8.8 | 84 | 9.3 | 80 | 8.4 | 85 | 9.8 | 86 | 9.5 |
| His | (H) | 14 | 1.5 | 14 | 1.6 | 13 | 1.4 | 16 | 1.9 | 13 | 1.6 | 11 | 1.3 | 16 | 1.8 | 14 | 1.5 | 16 | 1.8 | 12 | 1.3 |
| Ile | (I) | 53 | 5.5 | 49 | 5.5 | 51 | 5.7 | 53 | 6.4 | 41 | 5.0 | 38 | 4.3 | 51 | 5.6 | 44 | 4.6 | 35 | 4.0 | 44 | 4.9 |
| Leu | (L) | 95 | 9.9 | 68 | 7.6 | 71 | 7.9 | 62 | 7.4 | 60 | 7.3 | 66 | 7.5 | 67 | 7.4 | 80 | 8.4 | 74 | 8.5 | 68 | 7.5 |
| Lys | (K) | 26 | 2.7 | 29 | 3.2 | 44 | 4.9 | 29 | 3.5 | 49 | 5.9 | 51 | 5.8 | 40 | 4.4 | 30 | 3.2 | 50 | 5.8 | 56 | 6.2 |
| Met | (M) | 12 | 1.2 | 2 | 0.2 | 2 | 0.2 | 9 | 1.1 | 11 | 1.3 | 8 | 0.9 | 18 | 2.0 | 8 | 0.8 | 11 | 1.3 | 15 | 1.7 |
| Phe | (F) | 41 | 4.3 | 59 | 6.6 | 47 | 5.2 | 55 | 6.6 | 29 | 3.5 | 36 | 4.1 | 44 | 4.9 | 39 | 4.1 | 34 | 3.9 | 34 | 3.8 |
| Pro | (P) | 62 | 6.4 | 65 | 7.3 | 67 | 7.4 | 58 | 7.0 | 58 | 7.0 | 59 | 6.7 | 50 | 5.5 | 51 | 5.4 | 55 | 6.4 | 57 | 6.3 |
| ser | (S) | 77 | 8.0 | 69 | 7.7 | 91 | 10.1 | 60 | 7.2 | 66 | 8.0 | 70 | 8.0 | 72 | 7.9 | 111 | 11.7 | 63 | 7.3 | 61 | 6.7 |
| Thr | (T) | 87 | 9.0 | 89 | 10.0 | 75 | 8.3 | 83 | 10.0 | 65 | 7.9 | 73 | 8.3 | 71 | 7.8 | 72 | 7.6 | 71 | 8.2 | 68 | 7.5 |
| Trp | (W) | 5 | 0.5 | 3 | 0.3 | 6 | 0.7 | 2 | 0.2 | 12 | 1.5 | 12 | 1.4 | 12 | 1.3 | 9 | 0.9 | 12 | 1.4 | 14 | 1.5 |
| Tyr | (Y) | 33 | 3.4 | 12 | 1.3 | 26 | 2.9 | 7 | 0.8 | 29 | 3.5 | 28 | 3.2 | 39 | 4.3 | 50 | 5.3 | 26 | 3.0 | 27 | 3.0 |
| val | (V) | 76 | 7.9 | 82 | 9.2 | 80 | 8.9 | 66 | 7.9 | 57 | 6.9 | 74 | 8.4 | 58 | 6.4 | 68 | 7.2 | 82 | 9.5 | 62 | 6.8 |
|
| |||||||||||||||||||||
| Total | 963 | 100.0 | 893 | 100.0 | 900 | 100.0 | 834 | 100.0 | 825 | 100.0 | 878 | 100.0 | 906 | 100.0 | 950 | 100.0 | 866 | 100.0 | 906 | 100.0 | |
| MW | (Da) | 100989.8 | 93266.9 | 94436.5 | 86945.5 | 86954.9 | 92490.5 | 97934.3 | 100962.9 | 91720.9 | 96198.8 | ||||||||||
The accession numbers of proteases: putative serine protease of Glaciozymaantarctica PI12 (CAQ76821), subtilisin-like protease Pleurotus ostreatus (CAG25549), pyrolysin Laccaria bicolor S238N-H82 (EDR11679), subtilisin-like protease Phanerochaete chrysosporium (CAG38357), subtilisin-like serine protease PR1C Metarhizium anisopliae var. anisopliae (CAD11898), serine endopeptidase Hypocrea lixii (CAL25578), peptidase Pyrenophora tritici-repentis (EDU50747), peptidase Cryptococcus neoformans var. neoformans JEC21 (AAW40780), subtilisin-like protease Epichloe festucae (ACB30121), subtilisin-like protease Epichloe festucae (ACB30119).