Fig. 2.
Gel electrophoresis results of nested PCR of Leishmania parasites isolated from sandflies using the primers CSB1XR, CSB2XF, 13Z and LIR; marker (100-bp ladder) (lane 1), reference strain of Leishmania major (560 bp) (lane 2), reference strain of L. tropica (750 bp) (lane 3), reference strain of L. infantum (680 bp) (lane 4), a male sandfly as negative control (lane 5), L. major isolated from Phlebotomus salehi in Heydar-Abad (lane 6), L. major isolated from P. papatasi in Heydar-Abad (lane 7) and L. major isolated from P. papatasi in Mousavieh (lane 8).