Table 4.

Rates of reactivation after ethyl paraoxon inhibition measured for the DE variants at 37°C in 50 mM Tris pH 7.6, 150 mM NaCl, 2 mM BME.

Enzyme	CLONE	kreactivation (1/h)	% Reactivation
A107	D3	0.03 ± 0.01	110 ± 10
A107C	D4	0.15 ± 0.03	40 ± 3
A107D	D5	0.31 ± 0.02	90 ± 2
A107E	D6	0.048 ± 0.006	46 ± 4
A107F	D7	0.023 ± 0.004	130 ± 10
A107G	D8	0.0114 ± 0.0009	70 ± 4
A107H	–	0.53 ± 0.09	102 ± 5
A107I	D9	0.013 ± 0.004	70 ± 4
A107K	D10	0.04 ± 0.02	25 ± 6
A107L	D11	0.030 ± 0.005	25 ± 2
A107M	D12	0.06 ± 0.03	90 ± 10
A107N	E1	0.04 ± 0.01	60 ± 10
A107Q	E3	0.05 ± 0.02	110 ± 10
A107R	E4	0.14 ± 0.03	27 ± 2
A107S	E5	0.03 ± 0.01	100 ± 10
A107T	E6	0.034 ± 0.006	40 ± 5
A107V	E7	0.22 ± 0.03	28 ± 1
A107Y	E9	0.012 ± 0.003	7 ± 1
A107H/A190C	E10	1.5 ± 0.2a	62 ± 3
A107H/A190V	G2	0.4 ± 0.1	73 ± 9
A107H/A190G	F2	0.7 ± 0.3	90 ± 10
A107H/A190H	F3	0.10 ± 0.02	66 ± 8
A107H/A190M	F7	0.3 ± 0.2	17 ± 5
A107H/A400W	H10	0.4 ± 0.2	130 ± 50
A107H/A400M	H2	1.0 ± 0.2	97 ± 7
A107H/A400V	H9	0.6 ± 0.1	130 ± 20
A107H/A190C/A400T	A8	0.43 ± 0.07	92 ± 7
A107H/A190C/A400T	A8	1.0 ± 0.1a	75 ± 5
A107H/A190C/A400M	C4	1.0 ± 0.1a	75 ± 5

^aEnzymes were heated at 37°C prior to reactivation.