Table 4.

Intracellular expression of HMGB1 in U251MG cells after 24 and 48 hours from single or combined treatments with radiation and temozolomide (TMZ).

HMGB1	0 Gy	2 Gy	4 Gy	8 Gy	10 Gy	TMZ	2 Gy + TMZ	10 Gy + TMZ
24 h	+/++ (n)	++/+++ (p)	+ (n)	+++ (c)	+/++ (c)	++ (n)	+ (c)	+/++ (c)
48 h	+/++ (n)	++ (p)	+++ (p)	++/+++ (c)	+ (c)	++/+++ (p)	+ (c)	++ (p)

0: no expression of protein; +: low expression of protein; ++: moderate expression of protein; +++: high expression of protein; n: nuclear expression; p: perinuclear expression; c: cytoplasmatic expression.