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. 2014 Jul 1;2014:290182. doi: 10.1155/2014/290182

Figure 2.

Figure 2

HT-22 hippocampal neurons are committed to necrosis rather than apoptosis in response to TNF-α. (a) HT-22 hippocampal neuronal cells were treated as indicated for 20 h. Cell viability was determined by measuring ATP levels. Data are represented as mean ± standard deviation of duplicates. T: TNF-α; S: Smac mimetic (100 nM); and Z: z-VAD (20μM). (b) HT-22 hippocampal neurons were treated with DMSO or TNF-α (300 ng/mL)/z-VAD for 20 h and then analyzed for PI staining by flow cytometry. Identical concentrations were used in later experiments. Data are represented as mean ± standard deviation of duplicates.  *P < 0.01,  **P < 0.001 versus control. All experiments were repeated three times with similar results.