Figure 5.

MLKL is essential for TNF-α-induced necrosis of HT-22 cells. (a) HT-22 cells were transfected with the negative control or MLKL siRNAs. After 60 h, cells were treated with control or TNF-α/z-VAD for another 20 h and then cell viability was determined by measuring ATP levels. Data were represented as mean ± standard deviation of duplicates.  *P < 0.01,  **P < 0.001 versus NC-T + Z. (b) The knockdown efficiency of MLKL RNAi. Cell lysates were collected 60 h after transfection and subjected to western blot analysis of MLKL and β-actin levels. All experiments were repeated three times with similar results.