Figure 6.

TNF-α-induced necrosis of HT-22 cells is largely independent of ROS accumulation and calcium. (a) HT-22 cells were treated with control or BHA or NAC at the indicated concentration 3 h before the treatment of control or TNF-α/z-VAD for 20 h; cell viability was determined by measuring ATP levels.  *P < 0.01,  **P < 0.001 versus control-T + Z. (b) MEF cells were treated with control or BMS NAC at the indicated concentration 3 h before the treatment of control or TNF-α/Smac mimetic/z-VAD for 20 h; cell viability was determined by measuring ATP levels.  *P < 0.01,  **P < 0.001 versus control-T + S + Z. (c) HT-22 cells were treated with control or LaCl₃ or NAC at the indicated concentration 3 h before the treatment of control or TNF-α/z-VAD for 20 h; cell viability was determined by measuring ATP levels. Data are represented as mean ± standard deviation of duplicates.  *P < 0.01,  **P < 0.001 versus control-T + Z. All experiments were repeated three times with similar results.