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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Biochem J. 2014 May 15;460(1):59–67. doi: 10.1042/BJ20140014

Figure 1. Analysis of the ribosomal stalk in the yeast mutants.

Figure 1

(A) Schematic representation of the yeast stalk mutants. (B) Immunoblot analysis of the yeast stalk mutants. Ribosomes (10 pmol) and the cytosolic fraction (40 µg of total protein) isolated from the P0ΔH1(P1B–P2A), and P0ΔH2(P1A–P2B) mutants and the isogenic wild-type were analysed by immunoblot analysis using monoclonal antibody against the C-termini of P-proteins (IB3B) to detect the P0 protein. Monoclonal antibodies against P2A (IBE3) and P2B (IAA9) were used to detect P2A and P2B respectively. Anti-L3 and anti-Pgk1 antibodies were used as loading controls for the ribosome and cytosol fractions respectively. The immunoblot analysis was repeated three times using different ribosome preparations. (C) Growth of yeast stalk mutants on minimal medium supplemented with 2 % glucose.