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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Biochem J. 2014 May 15;460(1):59–67. doi: 10.1042/BJ20140014

Figure 3. Interaction of RTA with ribosomes from the stalk mutants.

Figure 3

(A) Interaction of RTA with ribosomes isolated from wild-type and stalk mutants was analysed with a Biacore T200 system at a 1 nM ribosome concentration. (B) Interaction of RTA with the stalk mutant ribosomes at a 1 nM concentration. (C) Interaction of RTA with the stalk mutant ribosomes at a 5 nM concentration. The interactions were measured using a Biacore T200 system with a CM3 chip. RTA was immobilized to Fc2 at 840 RU by amine coupling. Fc1 was activated and blocked as a control. Ribosomes from wild-type yeast or the stalk mutants were passed over both surfaces at 40 µl/min. Running buffer consisted of 10 mM Hepes, pH 7.6, 150 mM NaCl, 10 mM magnesium acetate, 5 µM EDTA and 0.005% surfactant P20.