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. Author manuscript; available in PMC: 2015 Jun 11.
Published in final edited form as: Cell Host Microbe. 2014 Jun 11;15(6):681–691. doi: 10.1016/j.chom.2014.05.010

Figure 4. Blocking access to cell-surface NgR1 diminishes reovirus infection of neurons.

Figure 4

Primary murine cortical neuron cultures prepared from (A) wildtype JAM-A+/+ or (B) isogenic JAM-A−/− embryos were pretreated with either PBS or antibodies specific for JAM-A or NgR1 prior to adsorption with reovirus T3SA+ at an MOI of 500 PFU/cell. (C) Neuron cultures prepared from wildtype cortices were treated with PI-PLC at increasing concentrations for 1 h prior to adsorption with reovirus T3SA+ at an MOI of 500 PFU/cell. (D) Reovirus T3SA+ was preincubated with increasing amounts of soluble Fc-tagged NgR1, JAM-A (30 µg/ml), or CAR (30 µg/ml) prior to inoculation of wildtype neuron cultures at an MOI of 500 PFU/cell. (A – D) Cells were fixed at 20 h and scored for reovirus antigen (green) using indirect immunofluorescence. Results are expressed as the mean FFU/field for three fields of view in triplicate samples. Representative images of an experiment in (D) are shown in (E). Error bars indicate SD. *, P < 0.05; **, P < 0.005; ***, P < 0.0005 (as determined by Student’s t test in comparison to PBS-treated infected cells). See also Figures S1 and S5.