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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Methods Mol Biol. 2012;920:221–243. doi: 10.1007/978-1-61779-998-3_16

Fig. 3.

Fig. 3

Examination of dual incisions during ICL repair. HincII digested repair intermediates are separated on a denaturing gel and visualized using Southern blotting (land 5–14) (Subheading 3.4). Undigested and HincII and/or SapI digested pControl and pICL are shown in lane 1–4 and serve as size markers. X-structures, linears, and 2.3/3.3 arm fragments are indicated. pQuant is visible due to some background reactivity with the probe.