Histological and biochemical analyses of fibrogenesis. A-C: Histological staining (HE staining) of control liver sections (A) and sections obtained on d 4 (B) and d 21 (C), respectively, after DMN administration; D: Biochemical analysis of fibrogenesis showing plasma AST levels (IU/L, solid line), plasma hyaluronic acid levels (ng/mL, dashed line), and liver hydroxyproline levels (ng/mL, dotted line). The x-axis shows the days of fibrogenesis, and each value on the graph is shown as the mean ± SE, n = 5.