Skip to main content
. 2014 Jul 16;9(7):e102066. doi: 10.1371/journal.pone.0102066

Figure 3. Suppression of LC3-II and ATG7 protein levels in CD4+CD8− and CD4−CD8+ T cells after CLP.

Figure 3

Splenic CD4+ and CD8+ T cell extracts were used for LC3-II and ATG7 protein expressions by Western blot analysis. CD4+ and CD8+ T cells obtained at 24h after CLP were isolated using CD4 and CD8 microbeads, respectively. A, Representative immunoblots of LC3-II and ATG7. B, Densitometric values of LC3-II. C, Densitometric values of ATG7. Actin was used as a loading control. Data are shown as mean ± SEM of 3 animals in each group and compared by two-tailed Student t-test. *P<0.05 vs. sham-operated mice.