Table 1.

O9 and Vi slide agglutination reactions of S. Typhi strains grown on LB agar (pH 7) supplemented with different amounts of NaCl at 37°C overnight (18–24 h).

	Environment				Blood-Fluid tissues
NaCl (mM)	0		10		85		150
Strains	O9	Vi	O9	Vi	O9	Vi	O9	Vi
Ty2 RpoS−	−	+++	−	+++	−	+++	−	+++
ISP RpoS+	++	++	+++	++	++	++	++	++
Ty2 RpoS+	++	++	+++	++	++	++	+++	++
Ty2 rpoSΩAp	−	+++	−	+++	−	+++	−	+++
ISP rpoSΩAp	−	+++	−	+++	−	+++	−	+++

	Intestinal lumen
NaCl (mM)	300		400		500
Strains	O9	Vi	O9	Vi	O9	Vi
Ty2 RpoS−	++	+++	+++	+	+++	−
ISP RpoS+	+++	−	+++	−	+++	−
Ty2 RpoS+	+++	−	+++	−	+++	−
Ty2 rpoSΩAp	++	+++	+++	+	+++	−
ISP rpoSΩAp	++	+++	+++	+	+++	−

O₉ agglutination reactions were carried out without prior boiling of cells. The degree of agglutinations ranged from not detectable (−) to weak (+) to strong (+++); ± and ++ indicate intermediate degrees. Adapted with permission from Santander et al. [51].