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. 2014 Jun 23;53(27):4368–4380. doi: 10.1021/bi500219t

Figure 5.

Figure 5

MftR binds both palindromes in its operator DNA, and the complexes are sensitive to urate. (A) EMSA showing mftpO (3.0 nM) titrated with increasing concentrations of MftR (0.1–200 nM; lanes 2–15); reaction in lane 1 contains DNA only. Complex and free DNA are identified at the right as C and F, respectively. (B) Fractional complex formation plotted as a function of MftR concentration. Binding isotherm with mftrO (○; solid line) and mftpO (+; dashed line). (C) Effect of urate on the binding of MftR to mftpO. Lane 1 contains DNA only. Reaction in lane 2 contains no ligand. The MftR-mftpO complex was titrated with increasing concentrations of urate (3–18 mM; lanes 3–11). (D) Normalized complex fraction as a function of urate concentration. MftR-mftrO complex (○; solid line) and MftR-mftpO complex (+; dashed line) titrated with increasing concentrations of urate. Error bars represent the standard deviation of three independent repeats.