Table 1. Thermal Stability of MftR and Mutants.
6 μM urate | 100 μM urate | mftrO | mftpO | ||
---|---|---|---|---|---|
MftR D1 | 49.4 ± 0.3 | 49.2 ± 0.2 | 61.3 ± 1.0 | 53.0 ± 0.2 | 52.7 ± 0.2 |
MftR D2 | 57.9 ± 0.1 | 58.0 ± 0.1 | 60.3 ± 0.1 | 60.9 ± 0.3 | |
W11F | 24.7 ± 0.1 | 27.0 ± 0.1 | 28.8 ± 0.7 | a | a |
D56S | 50.5 ± 0.2 | 52.3 ± 0.3 | 51.3 ± 0.1 | 48.4 ± 0.1 | 48.0 ± 0.1 |
R63S | 43.0 ± 0.1 | 48.7 ± 0.4 | 64.1 ± 3.9 | 42.8 ± 0.2 | 43.0 ± 0.2 |
R89N | 53.0 ± 0.2 | 55.9 ± 0.2 | 63.0 ± 0.6 | 54.0 ± 0.1 | 55.6 ± 0.9 |
Did not yield quantifiable data.