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. 2014 Jul 16;9(7):e102259. doi: 10.1371/journal.pone.0102259

Figure 4. miRNAs DE by cell type.

Figure 4

(A) The 10 most stable miRNAs across all cell types are shown for both NormFinder (left) and Coefficient of Variation (CV) methods (right). (B) Validation of NanoString-derived data for miR-301a-3pby qRT-PCR data. The X-axis represents the expression level of each miRNA normalized to miR-30c-3p using 2−ΔCt method. Each point represents the mean ± SEM of 5 subjects for each cell type. (C,D) Validation of microRNAs DE by cell type. The NanoString-derived data for the indicated miRNAs was validated by qRT-PCR using RNA isolated from the 5 hematopoietic cell types from two different preparations of cells and RNA. qRT-PCR data was normalized and presented as in Figure 4B.P, platelets; T, T-cells; B, B-cells; G, granulocytes; E, erythrocytes.