Table 1. Identification of mRNAs within islet-derived EVs by RT-PCR array.

Gene family	Gene	ΔCt	2∧-ΔCt	Gene family	Gene	ΔCt	2∧-ΔCt
Receptors, transporters and channels	ATP-binding cassette, sub-family C (CFTR/MRP), member 8	2.7	0.153	Secreted factors	Angiotensinogen (serpin peptidase inhibitor,clade A, member 8)	3.2	0.108
	Adrenergic, beta-3-, receptor	4	0.062		Chemokine (C-C motif) ligand 5	3.2	0.108
	Aquaporin 2 (collecting duct)	3	0.125		Glucagon	0.6	0.659
	Carcinoembryonic antigen-related cell adhesion molecule 1(biliary glycoprotein)	3	0.125		Interferon, gamma	3.1	0.116
	Glucagon-like peptide 1 receptor	2.3	0.203		Interleukin 6 (interferon, beta 2)	3.7	0.076
	Intercellular adhesion molecule 1	2	0.25		Interleukin 10	2.7	0.153
	Interleukin 4 receptor	2.2	0.217		Insulin	-1.7	3.249
	Insulin receptor	3.6	0.082		Resistin	2.3	0.203
	N-ethylmaleimide-sensitive factor	2.2	0.217		Tumor necrosis factor	2.8	0.143
	RAB4A, member RAS oncogene family	2.7	0.153		Vascular endothelial growth factor A	3.4	0.094
	Solute carrier family 2 (facilitated glucose transporter), member 4	3.1	0.116	Signal transduction	V-akt murine thymoma viral oncogene homolog 2	3.9	0.066
	Synaptosomal-associated protein, 23 kDa	3	0.125		Dual specificity phosphatase 4	3.2	0.108
	Synaptosomal-associated protein, 25 kDa	3	0.125		Inhibitor of kappa light polypeptide geneenhancer in B-cells, kinase beta	3	0.125
	Syntaxin 4	2.5	0.176		Inositol polyphosphate phosphatase-like 1	3.3	0.101
	Tumor necrosis factor receptor superfamily, member 1A	3.4	0.094		Insulin receptor substrate 2	3.2	0.108
	Vesicle-associated membrane protein 3 (cellubrevin)	3.2	0.108		Mitogen-activated protein kinase 8	2.6	0.164
	VAMP (vesicle-associated membrane protein)-associated protein A,33 kDa	2	0.125		Phosphoinositide-3-kinase, class 2, beta polypeptide	2.6	0.164
Nuclear receptors	Peroxisome proliferator-activated receptor alpha	2.5	0.176		Phosphoinositide-3-kinase, catalytic, delta polipeptide	2.4	0.189
	Peroxisome proliferator-activated receptor gamma	4	0.062		Phosphoinositide-3-kinase, regulatory subunit 1 (alpha)	2.6	0.164
Metabolic enzymes	ATP citrate lyase	2.3	0.203		Protein tyrosine phosphatase, non-receptor type 1	3.8	0.071
	Glucose-6-phosphatase, catalytic subunit	3	0.125		Tribbles homolog 3 (Drosophila)	3.1	0.116
	Glucokinase (hexokinase 4)	2.3	0.203	Transcriptional factors	CCAAT/enhancer binding protein (C/EBP), alpha	3.5	0.088
	Glycerol-3-phosphate dehydrogenase 1 (soluble)	2.3	0.203		Forkhead box C2 (MFH-1, mesenchyme forkhead 1)	3.3	0.101
	Glycogen synthase kinase 3 beta	2.9	0.133		Forkhead box G1	2.7	0.153
	Heme oxygenase (decycling) 1	3.3	0.101		Pancreatic and duodenal homeobox 1	2.4	0.189
	Insulin-degrading enzyme	3.4	0.094		Nuclear respiratory factor 1	2.9	0.133
	Malic enzyme 1, NADP(+)-dependent, cytosolic	2.6	0.164		Peroxisome proliferator-activated receptor gamma,coactivator 1 alpha	3	0.125
	Nitric oxide synthase 3 (endothelial cell)	2.4	0.189		Peroxisome proliferator-activated receptor gamma,coactivator 1 beta	3.1	0.116
	Poly (ADP-ribose) polymerase 1	3.7	0.076		Sterol regulatory element binding transcription factor 1	3.9	0.066
	Protein kinase, AMP-activated, alpha 1 catalytic subunit	3.4	0.094		HNF1 homeobox B	2.5	0.176
	Protein kinase C, beta	3.2	0.108		NK2 homeobox 1	2.7	0.153
	Phosphorylase, glycogen, liver	3.3	0.101

Values are expressed as ΔCt (gene Ct – actin Ct) and as 2∧-ΔCt (gene Ct – actin Ct).