Figure 7. Detection of the archaeal 16S rRNA gene in millipedes relative to millipede phylogeny.

The 16S rRNA gene, an indicator of methanogenic Archaea, was detected by PCR amplification (Table S1). Species belonging to framed groups were tested in this study. Red lines mark the lineages containing some positive species. In Polydesmida, only faecal pellets were tested. The phylogenetic tree was used according to Regier et al. [38].