Figure 3. No survival benefit for mice injected with DAPT treated GBM neurosphere cells. (A) WB showing expression of the Notch-1 receptor and the Notch transcriptional target Hes-1 in GBM neurosphere cells treated with DMSO for control (−) or 10 µM DAPT (+) for seven days, prior to intracranial injection. (B–D) Kaplan-Meyer plots showing the fractionated survival of mice injected with (B) 029, (C) 036, or (D) 048 cells pretreated for one week with either 10 μM DAPT or equal volumes of DMSO. P values represent the comparison of survival curves by log-rank test performed in the GraphPad Prism 4 software.