Figure 5.

qRT-PCR confirmation: flaxseed alters the “set point” of several miRNAs. Mature miRNAs and two structural small RNA controls (U6 and sno-135) were quantitated by qRT-PCR for samples from the four indicated groups. Data were processed by relative Cq method, with normalization to the geometric mean of all assayed RNAs. Kruskal–Wallis tests were performed with the Dunn post-hoc test. Statistical tests were done with relative Cq-level data before transformation to fold change relative to the control/control condition (control diet and no radiation). Shown are the resulting fold change values for five mature miRNAs. Indicated comparisons (*) were significant at α of 0.05.