Table 2. Characterization of the glioblastoma-derived cell primary cultures used in this study.

Primary cultures	MGMT/18s	MGMT protein	MGMT methylation	miR200a/U6	TMZ IC50, μM
GBM.4	0.21 ± 0.08 [2 105] *	+	M	1.1 10−2 ± 1.6 10−2	>300
GBM.5	7.0 10−3 ± 0.8 10−3 [5 103]	+	M	0.11 ± 0.51	>300
GBM.7	2.5 10−3 ± 0.7 10−3 [2.5 103]	+	UM	0.53	>300
GBM.8	5.0 10−3 ± 1.1 10−3 [5 103]	+/−	M		>300
GBM.9	2.1 10−4 ± 1.0 10−4 [2 102]	nd	M	1.12 ± 0.22	250
GBM.10	0.9 10−4 ± 0.3 10−4 [0.9 102]	nd	M		250
GBM.11	2.3 10−4 ± 0.9 10−4 [2.3 102]	nd	M	0.12	220
GBM.12	0.83 ± 0.03 [8 105]	++	UM	3.0 10−3 ± 0.6 10−3	>300
GBM.14	7.0 10−3 ± 2.3 10−2 [7 103]		UM	0.4 ± 2.1 10−2	220
GBM.15	0.80 ± 0.05 [8 105]	++	UM	1.33 10−3 ± 0.11 10−3	>300
GBM.16	5.0 10−2 ± 0.11 [5 104]	++	UM	1.50 10−2 ± 0.07 10−2	220
GBM.19	2.3 10−3 ± 0.6 10−3 [2.3 103]		UM	1.9 10−2 ± 0.7 10−3	>300
GBM.21	3.0 10−3 ± 0.9 10−4 [3 103]	++	UM	6.8 10−2 ± 0.8 10−2	>300
GBM.22	1.8 10−2 ± 1.0 10−4 [1.8 104]	++	UM	7.3 10−3 ± 1.6 10−3	>300
GBM.23	0.12 ± 0.05 [1.2 105]	++	UM		>300
GBM.24	1.9 10−3 ± 1.3 10−3 [1.9 103]		M	1.5 10−2 ± 2.1 10−2	>300
GBM.25	2.0 10−6 ± 0.3 10−6 [2]	nd	M	1.64 ± 0.11	180

*in brackets, relative expression level of MGMT, taking MGMT level in U87 cells as 1 (cf Table 1); nd, no detectable; M, methylated MGMT; UM, unmethylated MGMT.