Figure 3. HPLC analysis of mucous materials. Authentic levan and mucous material collected from colonies of WT, gumD, and lsdA cells grown for 1 wk at 30 °C on the indicated solid media were hydrolyzed and analyzed. The vertical axis of the chromatograph shows the relative peak level. The retention time (min) is shown on the horizontal axis. Arrows show the levan peak (15.3 min).