Figure 5.

Characterization of F4/80⁺ macrophages based on the CD11b^low and CD11b^high expression. Three days after sham or burn procedure, skin cells from WT or δ TCR^−/− mice were prepared and studied for F4/80⁺CD11b⁺ macrophage cell characterization using flow cytometry. [A] Gating strategy. F4/80⁺CD11b⁺ cells from the lymphocyte/myeloid cell gate of WT (Fig. 1A, Upper Panel) and δ TCR^−/− (Fig. 1A, Lower Panel) mice. Representative histograms are shown from sham, burn uninjured and burn injured skin cells. The numbers indicate the percentages of respective population as determined by flow cytometry. Data are mean ± SEM for 3–7 mice/group; * p<0.05 vs. uninjured skin of the respective WT or δ TCR^−/− mice. † p<0.05 vs. burn wound of the respective WT mice.