Figure 4.

Mean ± S.E.M. % DNA methylation levels of the Nr3c1 gene exon 17 promoter across the 17 CpG sites for mixed-sex male (open bars), single-sex male (solid bars,) mixed-sex female (horizontal striped bars) and single-sex female (diagonal striped bars) offspring for the hippocampus (A) and cerebellum (B). The mean across all 17 CpG sites is shown at the far right of each figure. There were no group differences in hippocampus. In cerebellum, males from mixed-sex litters show higher DNA methylation levels than single-sex males and mixed-sex females. * LGC × Sex, P<0.05.