Table 1.
Quantification of virus particles in HSV-1(F) infected Tor1a+/+ and Tor1a−/− MEFsa
| No.(%) of nuclear capsids | No. (%) of perinuclear virions | No. (%) of cytoplasmic virus particles | No. (%) of surface virions | Total No. (%)of counted particles | ||
|---|---|---|---|---|---|---|
| Naked capsids | Enveloped virions | |||||
| Tor1a+/+ | 867 (68.1) | 5 (0.4) | 181 (14.2) | 184 (14.4) | 37 (2.9) | 1274 (100) |
| Tor1a−/− | 307* (66.2) | 11 (2.4) | 58 (12.5) | 81 (17.5) | 7 (1.5) | 464 (100) |
a
TEM images of ten randomly selected cells with at least one capsid or margination of chromatin were taken and subsequently counted for virus particles.
*
In cases where boundaries between the nucleus and cytoplasm were diminished due to NE breakdown capsids were counted as nuclear when no obvious cytoplasmic structures were visible in capsids proximity.