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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Antiviral Res. 2014 May 20;108:48–55. doi: 10.1016/j.antiviral.2014.05.007

Fig. 2. Representative primary biochemical screening assays for the HBV RNaseH.

Fig. 2

A. The oligonucleotide-directed RNaseH assay. Internally radiolabeled RNA is bound to a complementary DNA oligonucleotide, and the RNaseH cleaves the RNA within the DNA:RNA heteroduplexes. RNA, black line; DNA, grey line; S, RNA substrate; P1 and P2, RNaseH cleavage products. B. Representative primary screening assay employing HBV genotype D RNaseH. DMSO, vehicle control. C. E. coli RNaseH activity was not affected by compound #1 in the oligonucleotide-directed cleavage assay under identical conditions.