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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Carcinog. 2014 Jan 16;54(9):698–706. doi: 10.1002/mc.22131

Figure 1.

Figure 1

The effect of PC knockdown on the HIF-1α expression in A253 and D562 cells as compared to that of CoCl2. A. The cells were treated 200 μM overnight or transfected with different PC SiRNAs for 2 days and the cell lysates were collected for Western blot analysis. CoCl2 treatment, as well as SiRNA transfections of PC7 and furin upregulated HIF-1α expression. GAPDH was used as loading control. B. RT-PCR analysis of the RNA samples extracted from cells with the same treatment by using different primers for PC7, furin, PACE4, HIF-1α and GAPDH. PC7, furin and PACE4 downregulation was achieved by the corresponding SiRNA transfections as compared with GAPDH control. C. The expression levels of HIF-1α protein (from Fig. 1A) and PC7, furin, PACE4 and HIF-1α mRNAs (from Fig. 1B) were quantified, and normalized to their corresponding GAPDH loading controls, and depicted as relative numbers with respect to the results from cells without CoCl2 and siRNA treatments (value set at 1). The left and right panels are for A253 and D562 respectively.