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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Carcinog. 2014 Jan 16;54(9):698–706. doi: 10.1002/mc.22131

Figure 2.

Figure 2

PC7 or furin can synergize with CoCl2 to regulate HIF-1α expression. A. The synergistic effect of PC SiRNA with CoCl2. The cells were first transfected with different SiRNAs for 1 day and then treated with CoCl2 for another day. The lysates were subjected to Western blot analysis for HIF-1α and GAPDH expression. B. The synergistic effect of PC SiRNA with the proteosome inhibitor MG-132. The cells were first transfected with different SiRNAs for 2 days and then treated with 2.5 μM of MG-132 for 30 min before harvest for Western blot analysis of HIF-1α and GAPDH.