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. 2014 Jun 12;20(3):357–364. doi: 10.1007/s12298-014-0243-5

Table 3.

Comparative analysis of contents of withaferin A in different plant parts of W. ashwagandha from in vitro (static and liquid), green house and field grown plants

Source of Plant material Tissue type (Organ) Treatments Withaferin A (mg/g DW)
Field grown plant Leaves - 12.75 ± 0.60
Roots - 0.82 ± 0.07
In vitro culture* Shoots MS + 5.0 μM (BA) and 1.0 μM (Kn) 14.4 ± 1.15
Roots MS + 2.0 μM (IBA) 0.83 ± 0.06
In vitro culture (liquid) ** Shoots MS + 5.0 μM (BA) and 1.0 μM (Kn) 14.82 ± 1.50
Roots MS + 2.0 μM (IBA) 0.945 ± 0.10
Green house*** Shoots MS + 5.0 μM (BA) and 1.0 μM (Kn) 14.6 ± 0.85
Roots MS + 2.0 μM (IBA) 0.835 ± 0.15

*Plants grown on MS agar medium

**Shoots and roots were transferred in flasks containing 50 ml liquid culture medium and harvested after five weeks

***Green house plants were grown in green house in poly bags