Table 3.
Source of Plant material | Tissue type (Organ) | Treatments | Withaferin A (mg/g DW) |
---|---|---|---|
Field grown plant | Leaves | - | 12.75 ± 0.60 |
Roots | - | 0.82 ± 0.07 | |
In vitro culture* | Shoots | MS + 5.0 μM (BA) and 1.0 μM (Kn) | 14.4 ± 1.15 |
Roots | MS + 2.0 μM (IBA) | 0.83 ± 0.06 | |
In vitro culture (liquid) ** | Shoots | MS + 5.0 μM (BA) and 1.0 μM (Kn) | 14.82 ± 1.50 |
Roots | MS + 2.0 μM (IBA) | 0.945 ± 0.10 | |
Green house*** | Shoots | MS + 5.0 μM (BA) and 1.0 μM (Kn) | 14.6 ± 0.85 |
Roots | MS + 2.0 μM (IBA) | 0.835 ± 0.15 |
*Plants grown on MS agar medium
**Shoots and roots were transferred in flasks containing 50 ml liquid culture medium and harvested after five weeks
***Green house plants were grown in green house in poly bags